5-Me-UTP 100mM Solution Amount/Package:1mL / centrifuge tube RNA cyclisation: circular RNA is

RNA cyclisation: circular RNA is produced by joining the two ends within the RNA molecule by T4 RNA ligase 1 (T4 Rnl1) or T4 RNA ligase 2 (T4 Rnl2) catalysing the joining of 5'-phosphate and 3'-hydroxyl groups at the RNA terminus

mix well and incubate 30min at 37°C

3 (2023): e202200169

" Proceedings of the National Academy of Sciences 97

Heating the solution of RNA prior to incubation with Vaccinia capping enzyme

5-Me-UTP 100mM Solution Amount/Package:1mL / centrifuge tube RNA cyclisation: circular RNA is5 methyluridine triphosphate (5mUTP) is a chemically modified UTP analog that supports production of methyl modified RNAs with reduced immunogenicity and improved stability. This 100 mM disodium salt solution provides 5mUTP in a highly soluble, ready to use form suitable for both routine laboratory applications and high consistency manufacturing environments. Applications Modified RNA Synthesis In vitro transcription of chemically modified mRNA with