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1-10μ M of Calcein-AM was used for dead cell staining to obtain an optimal working concentration that would not stain the cytoplasm
and the levels of p70 measured by bioassays are consistent with those measured using p70-specific immunoassays
Dilution factor Recovery (%) 1:1 111 1:2 92 1:4 101 1:8 94
or tissues around the eye (termed extranodal marginal zone B-cell lymphoma of the cited organ)
2 mL of 1 × PBS can be added
SMAD3 (phospho S423+S425) Antibody Duo Reagent 1-10μ M of Calcein-AM wasProduct Specification Application WB, ICC Reactivity Hu Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05% BSA, 0. 03% Proclin 300 Stability & Storage 12 months from date of receipt reconstitution, 20 C as supplied
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