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and HRP conjugate are sequentially added to microwells pre-coated with a Cluster of Differentiation 14 (CD14) capture antibody
TNF-α binds to the ubiquitous 55-60 kDa TNFRI and the hematopoietic cell-restricted 78-80 kDa TNFRII
CD36 is also expressed in various immune cells and has been shown to regulate autophagy
As a glycoprotein with high carbohydrate content
Negative result :
Mouse ATF4 ELISA Kit Size:96T and HRP conjugate are sequentiallyProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and