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Expression System HEK293 Molecular Weight 45-70kDa (Reducing) Purity >95% by SDS-PAGE Endotoxin <0
4 Preparation of enzyme conjugate working solution: before use 15 Minutes will 100× Concentrated enzyme conjugate in 1000×g Centrifugation 1 Minutes
Two trimers can be covalently linked to create a 180 kDa hexamer which associates into >300 kDa high molecular weight (HMW) Adiponectin
binds specific double-stranded DNA sequences
It is recommended that the substrate be divided and the reaction solution is freshly prepared from the substrate stock each time
UA-Glo® BRET Detection System Size:200assays Expression System HEK293 Molecular WeightProduct Specification Stability & Storage Dry ice transportation. Store away from light at 20C or below, long term storage (>3 months) at 80C is recommended, with a shelf life of 12 months. Background The UA Glo BRET Assay Kit includes UA Glo BRET Ligand 618 and UA Glo BRET Micro Luciferase Substrate. UA Glo BRET Ligand 618 can serve as an energy acceptor in NanoBRET experiments, while UA Glo BRET Micro Luciferase Substrate acts as a substrate for