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application dilution species ICFCM 1:500 null ICC 1:1000 null WB 1:1000-1:20000 null
Washing solution per well 300uL Then the wash liquid in the plate is aspirated off
It is essential for cytokine-dependent proliferation and survival of lymphocytes
After incubation for 1 hour
Rinse with water
Alexa Fluor® 647 Rat Anti-Mouse CD5 Antibody (S-R732) Size:100T application dilution species ICFCM 1:500Product Specification Host Rat Antigen CD5 Synonyms T cell surface glycoprotein CD5; Lymphocyte antigen 1 (Ly 1; Lyt 1); Ly 1; Cd5 Location Cell membrane Accession P13379 Clone Number S R732 Antibody Type Rat mAb Isotype IgG2a,k Application FCM Reactivity Ms Positive Sample BALB c mouse splenocytes Purification Protein G Concentration 0. 2 mg ml Conjugation Alexa Fluor 647 Physical Appearance Liquid Storage Buffer PBS, 1% BSA, 0. 3% Proclin 300