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Wash the collected cells three times with pre-chilled PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS
1EU/μg Conjugation Unconjugated Tags & Cleavage sites Thrombin cleave site Storage Buffer 20 mM Hepes
as it can bind to monomeric and immune complex (IC) IgG
It is primarily localized to the nuclei of monocytes and macrophages
is a member of a family of heat shock proteins originally classified by their molecular mass of 60 kDa
Human WND ELISA Kit Size:96T Wash the collected cells threeProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
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